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Molarity is best used in expressing EDTA concentration, because the reaction involves the complexation of one metal ion by one EDTA molecule or ion, whether the free acid form of EDTA or one of its more soluble Sodium salts is used. The general reaction involving the Disodium salt is:

EDTA2- + Mn+ → [EDTA-M]+n-2
where M = Ca, Mg, Cd, Mn, Ni, Al, Zn, Bi, Co, Cu, or other metal ion

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