Short Description::
Long Description::
Isopropyl-β-d-thiogalactopyranoside (IPTG) is a kind of nonmetabolizable analog of lactose widely used for heterogeneous gene expression in Escherichia coli system. Lactose, a natural inducer of β-galactosidase gene, is the most popular substitute for IPTG due to its low cost and nontoxicity.
Application::
Isopropyl β-d-1-thiogalactopyranoside is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
Dose::
When stored as a powder at 4°C or below, IPTG is stable for 5 years. It is significantly less stable in solution; Sigma recommends storage for no more than a month at room temperature.
Note::
In blue-white screen, IPTG is used together with X-gal. Blue-white screen allows colonies that have been transformed with the recombinant plasmid rather than a non-recombinant one to be identified in cloning experiments.
Technical Specifications::
Product remark::
Industrial Usages::
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Related Information::
In blue-white screen, IPTG is used together with X-gal. Blue-white screen allows colonies that have been transformed with the recombinant plasmid rather than a non-recombinant one to be identified in cloning experiments.
RXMARINE CHEMICAL TAG::