O PHTHALADEHYDE AR
O PHTHALADEHYDE AR
It is a dialdehyde and a member of benzaldehydes. A reagent that forms fluorescent conjugation products with primary amines. It is used for the detection of many biogenic amines, peptides, and proteins in nanogram quantities in body fluids.
Ortho-Phthalaldehyde (OPA) has been approved for high-level sterilization of heat-sensitive medical instruments and is increasingly being used as a replacement in the healthcare industry for glutaraldehyde, a known sensitizer.
The invention discloses application of 4-methoxyl ortho-phthalaldehyde in detection of ammonium and nitrogen in sea water and a detection method. The detection method includes that 1) sodium citrate solution, 4-methoxyl ortho-phthalaldehyde solution and sodium sulfite solution are added in ammonium and nitrogen working liquid in sequence, pH value of a system is regulated by sodium hydroxide solution, luminescence curves within a range of 390-550nm are detected in lambda ex=370nm after a certain reaction time, fluorescence intensity at the position of maximum emission wavelength is recorded, and a working curve of ammonium and nitrogen concentration and the fluorescence intensity at the position of the maximum emission wavelength; and 2) ammonium and nitrogen content in sample solution is quantified by the fluorescence intensity at the position of maximum emission wavelength according to the working curve.
The invention discloses application of 4-methoxyl ortho-phthalaldehyde in detection of ammonium and nitrogen in sea water and a detection method. The detection method includes that 1) sodium citrate solution, 4-methoxyl ortho-phthalaldehyde solution and sodium sulfite solution are added in ammonium and nitrogen working liquid in sequence, pH value of a system is regulated by sodium hydroxide solution, luminescence curves within a range of 390-550nm are detected in lambda ex=370nm after a certain reaction time, fluorescence intensity at the position of maximum emission wavelength is recorded, and a working curve of ammonium and nitrogen concentration and the fluorescence intensity at the position of the maximum emission wavelength; and 2) ammonium and nitrogen content in sample solution is quantified by the fluorescence intensity at the position of maximum emission wavelength according to the working curve.